Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MAU2

Cell type

Cell type Class
Neural
Cell type
hTERT RPE-1
Primary Tissue
Eye
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
RPE1 cell line
cell type
RPE1 cell line
antibody
Mau2
treatment
FBS -

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed as previously described (Komata, M., et al., Chromatin immunoprecipitation protocol for mammalian cells. Methods Mol Biol, 2014. 1164: p. 33-8.). Total RNA was extracted using TRIzol (Life Technologies) and Nucleospin RNA (Macherey-Nagel) following the manufacturers' instructions. ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (New England BioLabs).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
50674564
Reads aligned (%)
98.7
Duplicates removed (%)
8.7
Number of peaks
12642 (qval < 1E-05)

hg19

Number of total reads
50674564
Reads aligned (%)
97.8
Duplicates removed (%)
10.0
Number of peaks
12427 (qval < 1E-05)

Base call quality data from DBCLS SRA